Systematic evaluation of Drosophila CRISPR tools reveals safe and robust 1 alternatives to autonomous gene drives in basic research
نویسندگان
چکیده
Systematic evaluation of Drosophila CRISPR tools reveals safe and robust 1 alternatives to autonomous gene drives in basic research 2 3 4 Fillip Port*, §, Nadine Muschalik* and Simon L Bullock§ 5 6 7 Cell Biology Division, MRC-Laboratory of Molecular Biology, Francis Crick 8 Avenue, Cambridge CB2 0QH, UK 9 10 *, § These authors contributed equally 11 12 G3: Genes|Genomes|Genetics Early Online, published on May 20, 2015 as doi:10.1534/g3.115.019083
منابع مشابه
Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research
The Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR associated (CRISPR/Cas) technology allows rapid, site-specific genome modification in a wide variety of organisms . Proof-of-principle studies in Drosophila melanogaster have used various CRISPR/Cas tools and experimental designs, leading to significant uncertainty in the community about how to put this technology into practice...
متن کاملCRISPR with independent transgenes is a safe and robust alternative to autonomous gene drives in basic research
CRISPR/Cas technology allows rapid, site-specific genome modification in a wide variety of organisms. CRISPR components produced by integrated transgenes have been shown to mutagenise some genomic target sites in Drosophila melanogaster with high efficiency, but whether this is a general feature of this system remains unknown. Here, we systematically evaluate available CRISPR/Cas reagents and e...
متن کاملOptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.
The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system has emerged recently as a powerful method to manipulate the genomes of various organisms. Here, we report a toolbox for high-efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) expression plasmids. Systematic evalua...
متن کاملAn optimized CRISPR/Cas toolbox for efficient germline and somatic genome engineering in Drosophila
The type II CRISPR/Cas system has recently emerged as a powerful method to manipulate the genomes of various organisms. Here, we report a novel toolbox for high efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) expression plasmids. Systematic evaluation reveals Cas9 lines with ubiquitous or germline restricted patterns of...
متن کاملCRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line
Objective(s): Multidrug resistance (MDR) is a major obstacle in the successful chemotherapy of ovarian cancer. Inhibition of P-glycoprotein (P-gp), a member of ATP-binding cassette (ABC) transporters, is a well-known strategy to overcome MDR in cancer. The aim of this study was to investigate the efficiency and ability of CRISPR/Cas9 genome editing technology to knockdown ABCB1 gene expression ...
متن کامل